What is a phage or bacteriophage?
Phages are viruses that infect bacterial cells. There are several types of phages that have been used as vehicles for phage display including Ff filamentous phage, Lambda and T7. In phage display, the bacteriophages are used to construct different phage libraries as peptide libraries or antibody library
History of phage display
Phage display technology was first introduced in 1985 by George Smith. After that many advantages have been introduced including the way to construct a phage library like antibody library to select phage display antibodies.
Advantages of phage display
Phage display is a system for large scale study and selection of proteins, peptide and antibodies based on their binding affinity and specificity. One advantage of phage display is the enormous diversity of variant proteins that can be represented in a phage library. Phage display provides a means of rapidly screening large numbers of proteins against potential binding partners.
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Human antibody

Human antibody production service

Human antibody can be obtained to be used for therapeutic research aim for clinical applicationes. Such antibody can be generated by converting previously obtained antibodies from lab animal such as mouse (antibody humanization)and humanizing such antibodies using antibody humanization technique or by selected human antibodies from a human antibody phage display library or from another systen such yeast library, bacterial display library, ribosome display library, etc.

Hybridoma

Hybridoma overview

Hybridoma technology is the technology that used to obtain custom monoclonal antibodies. The hybridoma technology consists in inject a target antigen as for example a recombinant protein or synthetic peptide into a mouse to induce the antibody production in the mouse against the target antigen. If the mouse produce antibodies with a high titer, then the mouse can be sacrified and their spleens removed. The b-cells from the spleens are isolated and then use to fuse them with a myoloma cell line to inmortilize them by converting them into hybrydoma cell lines. An important part in the hybridoma technology is the dilution technique and screening strategy to isolate those b-cells clones that produces monoclonal antibodies against the target antigen.

Hybridoma technology service

We can produce custom monoclonal antibodies againts any type of antigen using the hybridoma technology. Please fill in our contact form for more information. Online inquiry

 

References

1.- Hybridoma technologies for antibody production. Tomita M, Tsumoto K. Immunotherapy. 2011 Mar;3(3):371-80. doi: 10.2217/imt.11.4. Review.

2.- Production, novel assay development and clinical applications of monoclonal antibodies. Chiarella P.Recent Pat Anticancer Drug Discov. 2011 May;6(2):258-67. Review.

3.- Mouse monoclonal antibodies in biological research: strategies for high-throughput production. Chiarella P, Fazio VM. Biotechnol Lett. 2008 Aug;30(8):1303-10. doi: 10.1007/s10529-008-9706-5. Epub 2008 Apr 17. Review.

 

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Industry Today (press release)
This report studies Hybridoma Media in Global market, especially in North America, China, Europe, Southeast Asia, Japan and India, with production, revenue, consumption, import and export in these regions, from 2012 to 2016, and forecast to 2022.

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