What is a phage or bacteriophage?
Phages are viruses that infect bacterial cells. There are several types of phages that have been used as vehicles for phage display including Ff filamentous phage, Lambda and T7. In phage display, the bacteriophages are used to construct different phage libraries as peptide libraries or antibody library
History of phage display
Phage display technology was first introduced in 1985 by George Smith. After that many advantages have been introduced including the way to construct a phage library like antibody library to select phage display antibodies.
Advantages of phage display
Phage display is a system for large scale study and selection of proteins, peptide and antibodies based on their binding affinity and specificity. One advantage of phage display is the enormous diversity of variant proteins that can be represented in a phage library. Phage display provides a means of rapidly screening large numbers of proteins against potential binding partners.
antibody production

Phage display library

Phage display library overview

Phage display library is a library of phages in which  antibodies, peptides or proteins are expressed on the surface of the virion, usually it is used the virion protein pIII or pVIII. The library has a large population of clones from which a potential binder can be selected againts a specific target.

 

Phage display library types

 

Antibody Phage display library.

Small fragments of antibodies such as sFv, Fab or DAb are expressed in fussion with pIII protein of the phages. There are naïve and immunized antibody phage display libraries. More information can be found in our antibody library page.

 

Peptide phage display library

Small peptides can be expressed on the surface of virion in fussion with the pIII or pVIII protein. More information can be found in our peptide library page.

Protein library

Larger peptide fragments or entire small proteins can be expressed in the surface of the virion in fussion with the pIII protein.